1WP9

Crystal structure of Pyrococcus furiosus Hef helicase domain

1WP9 の概要

• エントリーDOI: 10.2210/pdb1wp9/pdb
• 関連するPDBエントリー: 1j22 1j23 1j24 1j25
• 分子名称: ATP-dependent RNA helicase, putative, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: helicase, atpase, dna replication, dna repair, dna recombination, hydrolase
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 338595.97

構造登録者

Nishino, T.,Komori, K.,Tsuchiya, D.,Ishino, Y.,Morikawa, K. (登録日: 2004-08-31, 公開日: 2005-02-01, 最終更新日: 2024-05-29)

主引用文献

Nishino, T.,Komori, K.,Tsuchiya, D.,Ishino, Y.,Morikawa, K.
Crystal Structure and Functional Implications of Pyrococcus furiosus Hef Helicase Domain Involved in Branched DNA Processing
Structure, 13:143-153, 2005

PubMed Abstract: DNA and RNA frequently form various branched intermediates that are important for the transmission of genetic information. Helicases play pivotal roles in the processing of these transient intermediates during nucleic acid metabolism. The archaeal Hef helicase/ nuclease is a representative protein that processes flap- or fork-DNA structures, and, intriguingly, its C-terminal half belongs to the XPF/Mus81 nuclease family. Here, we report the crystal structure of the helicase domain of the Hef protein from Pyrococcus furiosus. The structure reveals a novel helical insertion between the two conserved helicase core domains. This positively charged extra region, structurally similar to the "thumb" domain of DNA polymerase, plays critical roles in fork recognition. The Hef helicase/nuclease exhibits sequence similarity to the Mph1 helicase from Saccharomyces cerevisiae; XPF/Rad1, involved in DNA repair; and a putative Hef homolog identified in mammals. Hence, our findings provide a structural basis for the functional mechanisms of this helicase/nuclease family.

PubMed: 15642269
DOI: 10.1016/j.str.2004.11.008

実験手法

X-RAY DIFFRACTION (2.9 Å)