1WGI

STRUCTURE OF INORGANIC PYROPHOSPHATASE

1WGI の概要

• エントリーDOI: 10.2210/pdb1wgi/pdb
• 分子名称: INORGANIC PYROPHOSPHATASE, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: pyrophosphate phosphohydrolase, hydrolase, manganese
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm: P00817
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 64670.50

構造登録者

Heikinheimo, P.,Goldman, A. (登録日: 1996-10-24, 公開日: 1997-11-19, 最終更新日: 2024-02-14)

主引用文献

Heikinheimo, P.,Lehtonen, J.,Baykov, A.,Lahti, R.,Cooperman, B.S.,Goldman, A.
The structural basis for pyrophosphatase catalysis.
Structure, 4:1491-1508, 1996

PubMed Abstract: Soluble inorganic pyrophosphatase (PPase), an essential enzyme central to phosphorus metabolism, catalyzes the hydrolysis of the phosphoanhydride bond in inorganic pyrophosphate. Catalysis requires divalent metal ions which affect the apparent pKas of the essential general acid and base on the enzyme, and the pKa of the substrate. Three to five metal ions are required for maximal activity, depending on pH and enzyme source. A detailed understanding of catalysis would aid both in understanding the nature of biological mechanisms of phosphoryl transfer, and in understanding the role of divalent cations. Without a high-resolution complex structure such a model has previously been unobtainable.

PubMed: 8994974
DOI: 10.1016/S0969-2126(96)00155-4

実験手法

X-RAY DIFFRACTION (2.2 Å)