1WFA

WINTER FLOUNDER ANTIFREEZE PROTEIN ISOFORM HPLC6 AT 4 DEGREES C

1WFA の概要

• エントリーDOI: 10.2210/pdb1wfa/pdb
• 関連するPDBエントリー: 1WFB
• 分子名称: ANTIFREEZE PROTEIN ISOFORM HPLC6 (2 entities in total)
• 機能のキーワード: ice binding protein, thermal hysteresis protein, antifreeze polypeptide
• 由来する生物種: Pseudopleuronectes americanus (winter flounder)
• 細胞内の位置: Secreted, extracellular space: P04002
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6484.98

構造登録者

Yang, D.S.C.,Sicheri, F. (登録日: 1995-04-03, 公開日: 1995-06-03, 最終更新日: 2024-11-13)

主引用文献

Sicheri, F.,Yang, D.S.
Ice-binding structure and mechanism of an antifreeze protein from winter flounder.
Nature, 375:427-431, 1995

PubMed Abstract: Antifreeze proteins provide fish with protection against the freezing effect of polar environments by binding to ice surfaces and inhibiting growth of ice crystals. We present the X-ray crystal structure at 1.5 A resolution of a lone alpha-helical antifreeze protein from winter flounder, which provides a detailed look at its ice-binding features. These consist of four repeated ice-binding motifs, the side chains of which are inherently rigid or restrained by pair-wise side-chain interactions to form a flat binding surface. Elaborate amino- and carboxy-terminal cap structures are also present, which explain the protein's rich alpha-helical content in solution. We propose an ice-binding model that accounts for the binding specificity of the antifreeze protein along the <0112> axes of the (2021) ice planes.

PubMed: 7760940
DOI: 10.1038/375427a0

実験手法

X-RAY DIFFRACTION (1.7 Å)