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1WDK

fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native2)

1WDK の概要
エントリーDOI10.2210/pdb1wdk/pdb
関連するPDBエントリー1WDL 1WDM
分子名称Fatty oxidation complex alpha subunit, 3-ketoacyl-CoA thiolase, ZINC ION, ... (8 entities in total)
機能のキーワードalpha2beta2 heterotetrameric complex, lyase, oxidoreductase-transferase complex, oxidoreductase/transferase
由来する生物種Pseudomonas fragi
詳細
細胞内の位置Cytoplasm (By similarity): P28790
タンパク質・核酸の鎖数4
化学式量合計242324.54
構造登録者
Ishikawa, M.,Tsuchiya, D.,Oyama, T.,Tsunaka, Y.,Morikawa, K. (登録日: 2004-05-17, 公開日: 2004-07-27, 最終更新日: 2024-03-13)
主引用文献Ishikawa, M.,Tsuchiya, D.,Oyama, T.,Tsunaka, Y.,Morikawa, K.
Structural basis for channelling mechanism of a fatty acid beta-oxidation multienzyme complex
Embo J., 23:2745-2754, 2004
Cited by
PubMed Abstract: The atomic view of the active site coupling termed channelling is a major subject in molecular biology. We have determined two distinct crystal structures of the bacterial multienzyme complex that catalyzes the last three sequential reactions in the fatty acid beta-oxidation cycle. The alpha2beta2 heterotetrameric structure shows the uneven ring architecture, where all the catalytic centers of 2-enoyl-CoA hydratase (ECH), L-3-hydroxyacyl-CoA dehydrogenase (HACD) and 3-ketoacyl-CoA thiolase (KACT) face a large inner solvent region. The substrate, anchored through the 3'-phosphate ADP moiety, allows the fatty acid tail to pivot from the ECH to HACD active sites, and finally to the KACT active site. Coupling with striking domain rearrangements, the incorporation of the tail into the KACT cavity and the relocation of 3'-phosphate ADP bring the reactive C2-C3 bond to the correct position for cleavage. The alpha-helical linker specific for the multienzyme contributes to the pivoting center formation and the substrate transfer through its deformation. This channelling mechanism could be applied to other beta-oxidation multienzymes, as revealed from the homology model of the human mitochondrial trifunctional enzyme complex.
PubMed: 15229654
DOI: 10.1038/sj.emboj.7600298
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.5 Å)
構造検証レポート
Validation report summary of 1wdk
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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