1WCO

The solution structure of the nisin-lipid II complex

「1UZT」から置き換えられました

1WCO の概要

• エントリーDOI: 10.2210/pdb1wco/pdb
• 関連するPDBエントリー: 1AJ1 1MQX 1MQY 1MQZ 1QOW 1W9N 2DDE 2KTN 2KTO
• 関連するBIRD辞書のPRD_ID: PRD_000200
• 分子名称: ALA-FGA-LYS-DAL-DAL PEPTIDE, Lantibiotic, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-N-acetyl-alpha-muramic acid, ... (4 entities in total)
• 機能のキーワード: peptide-antibiotic complex, lantibiotic, antimicrobial, bacteriocin, thioester, pore formation, pyrophosphate cage, food preservative, peptide/antibiotic
• 由来する生物種: MONARTHROPALPUS FLAVUS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4749.46

構造登録者

Hsu, S.-T.D.,Breukink, E.,Tischenko, E.,Lutters, M.A.G.,de Kruijff, B.,Kaptein, R.,Bonvin, A.M.J.J.,van Nuland, N.A.J. (登録日: 2004-11-19, 公開日: 2005-03-07, 最終更新日: 2024-05-01)

主引用文献

Hsu, S.T.,Breukink, E.,Tischenko, E.,Lutters, M.A.,de Kruijff, B.,Kaptein, R.,Bonvin, A.M.,van Nuland, N.A.
The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics.
Nat. Struct. Mol. Biol., 11:963-967, 2004

PubMed Abstract: The emerging antibiotics-resistance problem has underlined the urgent need for novel antimicrobial agents. Lantibiotics (lanthionine-containing antibiotics) are promising candidates to alleviate this problem. Nisin, a member of this family, has a unique pore-forming activity against bacteria. It binds to lipid II, the essential precursor of cell wall synthesis. As a result, the membrane permeabilization activity of nisin is increased by three orders of magnitude. Here we report the solution structure of the complex of nisin and lipid II. The structure shows a novel lipid II-binding motif in which the pyrophosphate moiety of lipid II is primarily coordinated by the N-terminal backbone amides of nisin via intermolecular hydrogen bonds. This cage structure provides a rationale for the conservation of the lanthionine rings among several lipid II-binding lantibiotics. The structure of the pyrophosphate cage offers a template for structure-based design of novel antibiotics.

PubMed: 15361862
DOI: 10.1038/nsmb830

実験手法

SOLUTION NMR