1W8X

Structural analysis of PRD1

Summary for 1W8X

• Entry DOI: 10.2210/pdb1w8x/pdb
• Related: 1CJD 1GW7 1GW8 1HB5 1HB7 1HB9 1HQN 1HX6
• Descriptor: MAJOR CAPSID PROTEIN (PROTEIN P3), PROTEIN P30, PROTEIN P31, ... (4 entities in total)
• Functional Keywords: virus, p3 major capsid protein, p30 tape measure, p31 penton protein, p16 membrane protein, virus/viral protein, icosahedral virus
• Biological source: ENTEROBACTERIA PHAGE PRD1; More
• Cellular location: Virion: P22535 P27391 P27384; Virion membrane ; Single-pass membrane protein : P27392
• Total number of polymer chains: 15
• Total formula weight: 557106.82

Authors

Abrescia, N.G.A.,Cockburn, J.J.B.,Grimes, J.M.,Sutton, G.C.,Diprose, J.M.,Butcher, S.J.,Fuller, S.D.,San Martin, C.,Burnett, R.M.,Stuart, D.I.,Bamford, D.H.,Bamford, J.K.H. (deposition date: 2004-10-01, release date: 2004-11-11, Last modification date: 2024-05-08)

Primary citation

Abrescia, N.G.A.,Cockburn, J.J.B.,Grimes, J.M.,Sutton, G.C.,Diprose, J.M.,Butcher, S.J.,Fuller, S.D.,San Martin, C.,Burnett, R.M.,Stuart, D.I.,Bamford, D.H.,Bamford, J.K.H.
Insights Into Assembly from Structural Analysis of Bacteriophage Prd1.
Nature, 432:68-, 2004

PubMed Abstract: The structure of the membrane-containing bacteriophage PRD1 has been determined by X-ray crystallography at about 4 A resolution. Here we describe the structure and location of proteins P3, P16, P30 and P31. Different structural proteins seem to have specialist roles in controlling virus assembly. The linearly extended P30 appears to nucleate the formation of the icosahedral facets (composed of trimers of the major capsid protein, P3) and acts as a molecular tape-measure, defining the size of the virus and cementing the facets together. Pentamers of P31 form the vertex base, interlocking with subunits of P3 and interacting with the membrane protein P16. The architectural similarities with adenovirus and one of the largest known virus particles PBCV-1 support the notion that the mechanism of assembly of PRD1 is scaleable and applies across the major viral lineage formed by these viruses.

PubMed: 15525981
DOI: 10.1038/NATURE03056

Experimental method

X-RAY DIFFRACTION (4.2 Å)