1W7Z

Crystal structure of the free (uncomplexed) Ecballium elaterium trypsin inhibitor (EETI-II)

Summary for 1W7Z

• Entry DOI: 10.2210/pdb1w7z/pdb
• Related: 1H9H 1H9I 2C4B 2ETI 2LET
• Descriptor: TRYPSIN INHIBITOR II, SODIUM ION, FORMIC ACID, ... (4 entities in total)
• Functional Keywords: squash seed inhibitor, cystein knot, ecballium elaterium, trypsin, protease inhibitor
• Biological source: ECBALLIUM ELATERIUM (SQUIRTING CUCUMBER)
• Cellular location: Secreted: P12071
• Total number of polymer chains: 8
• Total formula weight: 25364.61

Authors

Kraetzner, R.,Debreczeni, J.E.,Pape, T.,Kolmar, H.,Schneider, T.R.,Uson, I.,Scheldrick, G.M. (deposition date: 2004-09-14, release date: 2005-11-01, Last modification date: 2024-11-20)

Primary citation

Kraetzner, R.,Debreczeni, J.E.,Pape, T.,Schneider, T.R.,Wentzel, A.,Kolmar, H.,Scheldrick, G.M.,Uson, I.
Structure of Ecballium Elaterium Trypsin Inhibitor II (Eeti-II): A Rigid Molecular Scaffold
Acta Crystallogr.,Sect.D, 61:1255-, 2005

PubMed Abstract: The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.

PubMed: 16131759
DOI: 10.1107/S0907444905021207

Experimental method

X-RAY DIFFRACTION (1.67 Å)