1VTO

1.9 A RESOLUTION REFINED STRUCTURE OF TBP RECOGNIZING THE MINOR GROOVE OF TATAAAAG

1VTO の概要

• エントリーDOI: 10.2210/pdb1vto/pdb
• 分子名称: DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*A)-3'), DNA (5'-D(*TP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*C)-3'), TATA BINDING PROTEIN, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, double helix, kinked, transcription-dna complex, transcription/dna
• 由来する生物種: ARABIDOPSIS THALIANA (mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 59853.77

構造登録者

Kim, J.L.,Burley, S.K. (登録日: 1996-09-06, 公開日: 2011-07-13, 最終更新日: 2026-01-14)

主引用文献

Kim, J.L.,Burley, S.K.
1.9 A Resolution Refined Structure of TBP Recognizing the Minor Groove of TATAAAAG
Nat.Struct.Biol., 1:638-653, 1994

PubMed Abstract: The three-dimensional structure of a TATA box-binding protein (TBP) from Arabidopsis thaliana complexed with a fourteen base pair oligonucleotide bearing the Adenovirus major late promoter TATA element has been refined at 1.9 A resolution, giving a final crystallographic R-factor of 19.4%. Binding of the monomeric, saddle-shaped alpha/beta protein induces an unprecedented conformational change in the DNA. A detailed structural and functional analysis of this unusual protein-DNA complex is presented, with particular emphasis on the mechanisms of DNA deformation, TATA element recognition, and preinitiation complex assembly.

PubMed: 7634103

実験手法

X-RAY DIFFRACTION (1.9 Å)