1VTE

MOLECULAR STRUCTURE OF NICKED DNA. MODEL A4

1VTE の概要

• エントリーDOI: 10.2210/pdb1vte/pdb
• 関連するPDBエントリー: 1NDN
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*AP*AP*AP*AP*CP*GP*CP*G)-3'), DNA (5'-D(*CP*GP*CP*GP*TP*T)-3'), DNA (5'-D(*TP*TP*CP*GP*CP*G)-3'), ... (4 entities in total)
• 機能のキーワード: b-dna, double helix, nicked, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 7281.83

構造登録者

Aymani, J.,Coll, M.,Van Der Marel, G.A.,Van Boom, J.H.,Wang, A.H.-J.,Rich, A. (登録日: 1990-05-21, 公開日: 2011-07-13, 最終更新日: 2023-12-27)

主引用文献

Aymami, J.,Coll, M.,van der Marel, G.A.,van Boom, J.H.,Wang, A.H.,Rich, A.
Molecular structure of nicked DNA: a substrate for DNA repair enzymes.
Proc. Natl. Acad. Sci. U.S.A., 87:2526-2530, 1990

PubMed Abstract: The molecular structure of a nicked dodecamer DNA double helix, made of a ternary system containing d(CGCGAAAACGCG) + d(CGCGTT) + d(TTCGCG) oligonucleotides, has been determined by x-ray diffraction analysis at 3 A resolution. The molecule adopts a B-DNA conformation, not unlike those found in intact dodecamer DNA molecules crystallized in a somewhat different crystal lattice, despite a gap due to the absence of a phosphate group in the molecule. The helix has a distinct narrow minor groove near the center of the molecule at the AAAA region. This suggests that the internal stabilizing forces due to base stacking and hydrogen-bonding interactions are sufficient to overcome the loss of connectivity associated with the disruption of the covalent backbone of DNA.

PubMed: 2320572

実験手法

X-RAY DIFFRACTION (3 Å)