1VQA

GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ALA 35 AND ILE 47 REPLACED BY LEU 47 (V35A, I47L)

1VQA の概要

• エントリーDOI: 10.2210/pdb1vqa/pdb
• 分子名称: GENE V PROTEIN (2 entities in total)
• 機能のキーワード: dna-binding protein, mutant, gene v, dna binding protein
• 由来する生物種: Enterobacteria phage f1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9671.16

構造登録者

Skinner, M.M.,Terwilliger, T.C. (登録日: 1996-08-14, 公開日: 1997-02-12, 最終更新日: 2024-02-14)

主引用文献

Skinner, M.M.,Terwilliger, T.C.
Potential use of additivity of mutational effects in simplifying protein engineering.
Proc.Natl.Acad.Sci.USA, 93:10753-10757, 1996

PubMed Abstract: The problem of rationally engineering protein molecules can be simplified where effects of mutations on protein function are additive. Crystal structures of single and double mutants in the hydrophobic core of gene V protein indicate that structural and functional effects of core mutations are additive when the regions structurally influenced by the mutations do not substantially overlap. These regions of influence can provide a simple basis for identifying sets of mutations that will show additive effects.

PubMed: 8855252
DOI: 10.1073/pnas.93.20.10753

実験手法

X-RAY DIFFRACTION (1.8 Å)