1VIW

TENEBRIO MOLITOR ALPHA-AMYLASE-INHIBITOR COMPLEX

1VIW の概要

• エントリーDOI: 10.2210/pdb1viw/pdb
• 分子名称: ALPHA-AMYLASE, ALPHA-AMYLASE-INHIBITOR, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: complex (glycosidase-inhibitor), hydrolase, lectin, insect alpha-amylase, inhibitors, complex (glycosidase-inhibitor) complex, complex (glycosidase/inhibitor)
• 由来する生物種: Tenebrio molitor (yellow mealworm); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74872.06

構造登録者

Nahoum, V.,Egloff, M.P.,Payan, F. (登録日: 1998-07-21, 公開日: 1999-07-22, 最終更新日: 2024-10-30)

主引用文献

Nahoum, V.,Farisei, F.,Le-Berre-Anton, V.,Egloff, M.P.,Rouge, P.,Poerio, E.,Payan, F.
A plant-seed inhibitor of two classes of alpha-amylases: X-ray analysis of Tenebrio molitor larvae alpha-amylase in complex with the bean Phaseolus vulgaris inhibitor.
Acta Crystallogr.,Sect.D, 55:360-362, 1999

PubMed Abstract: The alpha-amylase from Tenebrio molitor larvae (TMA) has been crystallized in complex with the alpha-amylase inhibitor (alpha-AI) from the bean Phaseolus vulgaris. A molecular-replacement solution of the structure was obtained using the refined pig pancreatic alpha-amylase (PPA) and alpha-AI atomic coordinates as starting models. The structural analysis showed that although TMA has the typical structure common to alpha-amylases, large deviations from the mammalian alpha-amylase models occur in the loops. Despite these differences in the interacting loops, the bean inhibitor is still able to inhibit both the insect and mammalian alpha-amylase.

PubMed: 10089450
DOI: 10.1107/S0907444998010701

実験手法

X-RAY DIFFRACTION (3 Å)