1VCR

An icosahedral assembly of light-harvesting chlorophyll a/b protein complex from pea thylakoid membranes

1VCR の概要

• エントリーDOI: 10.2210/pdb1vcr/pdb
• 分子名称: Chlorophyll a-b binding protein AB80, CHLOROPHYLL A, CHLOROPHYLL B (3 entities in total)
• 機能のキーワード: lhc-ii, icosahedron, chlorophyll, antenna, photosystem, photosynthesis
• 由来する生物種: Pisum sativum (pea)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35727.90

構造登録者

Hino, T.,Kanamori, E.,Shen, J.-R.,Kouyama, T. (登録日: 2004-03-10, 公開日: 2004-03-30, 最終更新日: 2024-04-03)

主引用文献

Hino, T.,Kanamori, E.,Shen, J.R.,Kouyama, T.
An icosahedral assembly of the light-harvesting chlorophyll a/b protein complex from pea chloroplast thylakoid membranes.
Acta Crystallogr.,Sect.D, 60:803-809, 2004

PubMed Abstract: When the light-harvesting chlorophyll a/b protein complex (LHC-II) from pea thylakoid membranes is co-crystallized with native lipids, an octahedral crystal that exhibits no birefringence is obtained. Cryogenic electron micrographs of a crystal edge showed the crystal to be made up of hollow spherical assemblies with a diameter of 250 A. X-ray diffraction data at 9.5 A resolution revealed the spherical shell of LHC-II to have icosahedral symmetry. A T = 1 icosahedral model of LHC-II, in which the stromal surface of the protein faces outward, was constructed using the previously reported structure of the LHC-II trimer [Kühlbrandt et al. (1994), Nature (London), 367, 614-621]. The present result shows the first example of a well ordered three-dimensional crystal of icosahedral proteoliposomes.

PubMed: 15103124
DOI: 10.1107/S0907444904003233

実験手法

X-RAY DIFFRACTION (9.5 Å)