1VBK

Crystal structure of PH1313 from Pyrococcus horikoshii Ot3

Summary for 1VBK

• Entry DOI: 10.2210/pdb1vbk/pdb
• Descriptor: hypothetical protein PH1313, (4R)-2-METHYLPENTANE-2,4-DIOL (3 entities in total)
• Functional Keywords: pyrococcus horikoshii, structural genomics, riken structural genomics/proteomics initiative, rsgi, nppsfa, national project on protein structural and functional analyses, unknown function
• Biological source: Pyrococcus horikoshii
• Total number of polymer chains: 2
• Total formula weight: 70877.16

Authors

Sugahara, M.,Kunishima, N.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2004-02-27, release date: 2005-04-19, Last modification date: 2023-12-27)

Primary citation

Sugahara, M.,Murai, S.,Sugahara, M.,Kunishima, N.
Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3
Acta Crystallogr.,Sect.F, 63:56-58, 2007

PubMed Abstract: The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3 has been overexpressed and purified. Crystallization was performed by the oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray diffraction data set at 1.9 A resolution and a single anomalous dispersion data set from a selenomethionine-derivative crystal at 2.1 A resolution were collected using synchrotron radiation at 100 K. The native crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.7, b = 71.2, c = 141.8 A.

PubMed: 17183176
DOI: 10.1107/S1744309106054509

Experimental method

X-RAY DIFFRACTION (1.9 Å)