1VA5

Antigen 85C with octylthioglucoside in active site

1VA5 の概要

• エントリーDOI: 10.2210/pdb1va5/pdb
• 関連するPDBエントリー: 1DQY 1DQZ 1F0N 1F0P
• 分子名称: Antigen 85-C, octyl 1-thio-beta-D-glucopyranoside (3 entities in total)
• 機能のキーワード: alpha-beta hydrolase, structural genomics, psi, protein structure initiative, tb structural genomics consortium, tbsgc, transferase
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Secreted: P0A4V4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67575.18

構造登録者

Ronning, D.R.,Vissa, V.,Besra, G.S.,Belisle, J.T.,Sacchettini, J.C.,TB Structural Genomics Consortium (TBSGC) (登録日: 2004-02-11, 公開日: 2004-07-06, 最終更新日: 2023-10-25)

主引用文献

Ronning, D.R.,Vissa, V.,Besra, G.S.,Belisle, J.T.,Sacchettini, J.C.
Mycobacterium tuberculosis Antigen 85A and 85C Structures Confirm Binding Orientation and Conserved Substrate Specificity
J.Biol.Chem., 279:36771-36777, 2004

PubMed Abstract: The maintenance of the highly hydrophobic cell wall is central to the survival of Mycobacterium tuberculosis within its host environment. The antigen 85 proteins (85A, 85B, and 85C) of M. tuberculosis help maintain the integrity of the cell wall 1) by catalyzing the transfer of mycolic acids to the cell wall arabinogalactan and 2) through the synthesis of trehalose dimycolate (cord factor). Additionally, these secreted proteins allow for rapid invasion of alveolar macrophages via direct interactions between the host immune system and the invading bacillus. Here we describe two crystal structures: the structure of antigen 85C co-crystallized with octylthioglucoside as substrate, resolved to 2.0 A, and the crystal structure of antigen 85A, which was solved at a resolution of 2.7 A. The structure of 85C with the substrate analog identifies residues directly involved in substrate binding. Elucidation of the antigen 85A structure, the last of the three antigen 85 homologs to be solved, shows that the active sites of the three antigen 85 proteins are virtually identical, indicating that these share the same substrate. However, in contrast to the high level of conservation within the substrate-binding site and the active site, surface residues disparate from the active site are quite variable, indicating that three antigen 85 enzymes are needed to evade the host immune system.

PubMed: 15192106
DOI: 10.1074/jbc.M400811200

実験手法

X-RAY DIFFRACTION (2.02 Å)