1V7X

Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate

Summary for 1V7X

• Entry DOI: 10.2210/pdb1v7x/pdb
• Related: 1V7V 1V7W
• Descriptor: chitobiose phosphorylase, 2-acetamido-2-deoxy-alpha-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: beta-sandwich, (alpha/alpha)6 barrel, transferase
• Biological source: Vibrio proteolyticus
• Total number of polymer chains: 1
• Total formula weight: 91904.59

Authors

Hidaka, M.,Honda, Y.,Nirasawa, S.,Kitaoka, M.,Hayashi, K.,Wakagi, T.,Shoun, H.,Fushinobu, S. (deposition date: 2003-12-24, release date: 2004-06-22, Last modification date: 2023-12-27)

Primary citation

Hidaka, M.,Honda, Y.,Kitaoka, M.,Nirasawa, S.,Hayashi, K.,Wakagi, T.,Shoun, H.,Fushinobu, S.
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold.
Structure, 12:937-947, 2004

PubMed Abstract: Vibrio proteolyticus chitobiose phosphorylase (ChBP) belongs to glycosyl transferase family 36 (GT-36), and catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. As the first known structures of a GT-36 enzyme, we determined the crystal structure of ChBP in a ternary complex with GlcNAc and SO(4). It is also the first structures of an inverting phosphorolytic enzyme in a complex with a sugar and a sulfate ion, and reveals a pseudo-ternary complex structure of enzyme-sugar-phosphate. ChBP comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain, constituting a distinctive structure among GT families. Instead, it shows significant structural similarity with glycoside hydrolase (GH) enzymes, glucoamylases (GH-15), and maltose phosphorylase (GH-65) in clan GH-L. The structural similarity reported here, together with distant sequence similarities between ChBP and GHs, led to the reclassification of family GT-36 into a novel GH family, namely GH-94.

PubMed: 15274915
DOI: 10.1016/j.str.2004.03.027

Experimental method

X-RAY DIFFRACTION (2 Å)