1V7U

Crystal structure of Undecaprenyl Pyrophosphate Synthase with farnesyl pyrophosphate

1V7U の概要

• エントリーDOI: 10.2210/pdb1v7u/pdb
• 関連するPDBエントリー: 1JP3 1UEH
• 分子名称: Undecaprenyl pyrophosphate synthetase, FARNESYL DIPHOSPHATE (3 entities in total)
• 機能のキーワード: prenyltransferase, farnesyl pyrophosphate, isopentenyl pyrophosphate, substrate binding, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58109.23

構造登録者

Chang, S.-Y.,Ko, T.-P.,Chen, A.P.-C.,Wang, A.H.-J.,Liang, P.-H. (登録日: 2003-12-24, 公開日: 2004-01-13, 最終更新日: 2023-12-27)

主引用文献

Chang, S.-Y.,Ko, T.-P.,Chen, A.P.-C.,Wang, A.H.-J.,Liang, P.-H.
Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies
Protein Sci., 13:971-978, 2004

PubMed Abstract: Undecaprenyl pyrophosphate synthase (UPPs) catalyzes eight consecutive condensation reactions of farnesyl pyrophosphate (FPP) with isopentenyl pyrophosphate (IPP) to form a 55-carbon long-chain product. We previously reported the crystal structure of the apo-enzyme from Escherichia coli and the structure of UPPs in complex with sulfate ions (resembling pyrophosphate of substrate), Mg(2+), and two Triton molecules (product-like). In the present study, FPP substrate was soaked into the UPPs crystals, and the complex structure was solved. Based on the crystal structure, the pyrophosphate head group of FPP is bound to the backbone NHs of Gly29 and Arg30 as well as the side chains of Asn28, Arg30, and Arg39 through hydrogen bonds. His43 is close to the C2 carbon of FPP and may stabilize the farnesyl cation intermediate during catalysis. The hydrocarbon moiety of FPP is bound with hydrophobic amino acids including Leu85, Leu88, and Phe89, located on the alpha3 helix. The binding mode of FPP in cis-type UPPs is apparently different from that of trans-type and many other prenyltransferases which utilize Asprich motifs for substrate binding via Mg(2+). The new structure provides a plausible mechanism for the catalysis of UPPs.

PubMed: 15044730
DOI: 10.1110/ps.03519904

実験手法

X-RAY DIFFRACTION (2.35 Å)