1V6P
Crystal structure of Cobrotoxin
1V6P の概要
エントリーDOI | 10.2210/pdb1v6p/pdb |
分子名称 | Cobrotoxin, COPPER (II) ION, CHLORIDE ION, ... (6 entities in total) |
機能のキーワード | atomic resolution, short-chain neurotoxin, naja atra, copper ion, toxin |
由来する生物種 | Naja atra (Chinese cobra) |
細胞内の位置 | Secreted: P60770 |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 14871.49 |
構造登録者 | |
主引用文献 | Lou, X.,Liu, Q.,Tu, X.,Wang, J.,Teng, M.,Niu, L.,Schuller, D.J.,Huang, Q.,Hao, Q. The atomic resolution crystal structure of atratoxin determined by single wavelength anomalous diffraction phasing J.Biol.Chem., 279:39094-39104, 2004 Cited by PubMed Abstract: By using single wavelength anomalous diffraction phasing based on the anomalous signal from copper atoms, the crystal structure of atratoxin was determined at the resolution of 1.5 A and was refined to an ultrahigh resolution of 0.87 A. The ultrahigh resolution electron density maps allowed the modeling of 38 amino acid residues in alternate conformations and the location of 322 of 870 possible hydrogen atoms. To get accurate information at the atomic level, atratoxin-b (an analog of atratoxin with reduced toxicity) was also refined to an atomic resolution of 0.92 A. By the sequence and structural comparison of these two atratoxins, Arg(33) and Arg(36) were identified to be critical to their varied toxicity. The effect of copper ions on the distribution of hydrogen atoms in atratoxin was discussed, and the interactions between copper ions and protein residues were analyzed based on a statistical method, revealing a novel pentahedral copper-binding motif. PubMed: 15252034DOI: 10.1074/jbc.M403863200 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (0.87 Å) |
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