1V6J

peanut lectin-lactose complex crystallized in orthorhombic form at acidic pH

1V6J の概要

• エントリーDOI: 10.2210/pdb1v6j/pdb
• 関連するPDBエントリー: 1CR7 1V6I 1V6K 1V6L 1V6M 1V6N 1V6O 2PEL
• 関連するBIRD辞書のPRD_ID: PRD_900008
• 分子名称: Galactose-binding lectin, beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: lectin, open quaternary association, orthorhombic, carbohydrate specificity, protein crystallography, agglutinin, sugar binding protein
• 由来する生物種: Arachis hypogaea (peanut)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 100574.79

構造登録者

Kundhavai Natchiar, S.,Arockia Jeyaprakash, A.,Ramya, T.N.C.,Thomas, C.J.,Suguna, K.,Surolia, A.,Vijayan, M. (登録日: 2003-12-01, 公開日: 2004-02-10, 最終更新日: 2023-12-27)

主引用文献

Kundhavai Natchiar, S.,Arockia Jeyaprakash, A.,Ramya, T.N.,Thomas, C.J.,Suguna, K.,Surolia, A.,Vijayan, M.
Structural plasticity of peanut lectin: an X-ray analysis involving variation in pH, ligand binding and crystal structure.
Acta Crystallogr.,Sect.D, 60:211-219, 2004

PubMed Abstract: Until recently, it has only been possible to grow crystals of peanut lectin when complexed with sugar ligands. It is now shown that it is possible to grow peanut lectin crystals at acidic pH in the presence of oligopeptides corresponding to a loop in the lectin molecule. Crystals have also been prepared in the presence of these peptides as well as lactose. Low-pH crystal forms of the lectin-lactose complex similar to those obtained at neutral pH have also been grown. Thus, crystals of peanut lectin grown under different environmental conditions, at two pH values with and without sugar bound to the lectin, are now available. They have been used to explore the plasticity and hydration of the molecule. A detailed comparison between different structures shows that the lectin molecule is sturdy and that the effect of changes in pH, ligand binding and environment on it is small. The region involving the curved front beta-sheet and the loops around the second hydrophobic core is comparatively rigid. The back beta-sheet involved in quaternary association, which exhibits considerable variability, is substantially flexible, as is the sugar-binding region. The numbers of invariant water molecules in the hydration shell are small and they are mainly involved in metal coordination or in stabilizing unusual structural features. Small consistent movements occur in the combining site upon sugar binding, although the site is essentially preformed.

PubMed: 14747696
DOI: 10.1107/S090744490302849X

実験手法

X-RAY DIFFRACTION (2.9 Å)