1V34

Crystal structure of Pyrococcus horikoshii DNA primase-UTP complex

Summary for 1V34

• Entry DOI: 10.2210/pdb1v34/pdb
• Related: 1V33
• Descriptor: DNA primase small subunit, ZINC ION, URIDINE 5'-TRIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: nucleotidyl transferase, riken structural genomics/proteomics initiative, rsgi, structural genomics, transferase
• Biological source: Pyrococcus horikoshii
• Total number of polymer chains: 1
• Total formula weight: 43192.63

Authors

Ito, N.,Nureki, O.,Shirouzu, M.,Yokoyama, S.,Hanaoka, F.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2003-10-25, release date: 2004-03-23, Last modification date: 2023-12-27)

Primary citation

Ito, N.,Nureki, O.,Shirouzu, M.,Yokoyama, S.,Hanaoka, F.
Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis.
Genes Cells, 8:913-923, 2003

PubMed Abstract: In chromosomal DNA replication, DNA primase initiates the synthesis of a dinucleotide on a single-stranded template DNA, and elongates it to form a primer RNA for the replicative DNA polymerase. Although the apo-structure of an archaeal primase has been reported, the mechanism of primer synthesis by the eukaryotic-type primase still remains to be elucidated.

PubMed: 14750947
DOI: 10.1111/j.1365-2443.2003.00693.x

Experimental method

X-RAY DIFFRACTION (2.7 Å)