1V29

Crystal structure of Nitrile hydratase from a thermophile Bacillus smithii

1V29 の概要

• エントリーDOI: 10.2210/pdb1v29/pdb
• 関連するPDBエントリー: 1AHJ 1IRE 2AHJ
• 分子名称: nitrile hydratase a chain, nitrile hydratase b chain, COBALT (II) ION, ... (4 entities in total)
• 機能のキーワード: nhase, bacillus smithii, lyase
• 由来する生物種: Bacillus smithii; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52175.24

構造登録者

Hourai, S.,Miki, M.,Takashima, Y.,Mitsuda, S.,Yanagi, K. (登録日: 2003-10-09, 公開日: 2004-10-09, 最終更新日: 2023-12-27)

主引用文献

Hourai, S.,Miki, M.,Takashima, Y.,Mitsuda, S.,Yanagi, K.
Crystal structure of nitrile hydratase from a thermophilic Bacillus smithii
Biochem.Biophys.Res.Commun., 312:340-345, 2003

PubMed Abstract: The crystal structure of the nitrile hydratase (NHase) from Bacillus smithii SC-J05-1 was determined. Our analysis of the structure shows that some residues that seem to be responsible for substrate recognition are different from those of other NHases. In particular, the Phe52 in the beta subunit of NHase from B. smithii covers the metal center partially like a small lid and narrows the active site cleft. It is well known that the NHase from B. smithii especially prefers aliphatic nitriles for its substrate rather than aromatic ones, and we can now infer that the Phe52 residue may play a key role in the substrate specificity for this enzyme. This finding leads us to suggest that substitution of these residues may alter the substrate specificity of the enzyme.

PubMed: 14637142
DOI: 10.1016/j.bbrc.2003.10.124

実験手法

X-RAY DIFFRACTION (2.6 Å)