1V1G

Structure of the Arabidopsis thaliana SOS3 complexed with Calcium(II) ion

1V1G の概要

• エントリーDOI: 10.2210/pdb1v1g/pdb
• 関連するPDBエントリー: 1V1F
• 分子名称: CALCINEURIN B-LIKE PROTEIN 4, IODIDE ION, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: signalling protein, salt stress response in plants, calcineurin b-like protein, protein crystallography, calcium sensor, ef-hand
• 由来する生物種: ARABIDOPSIS THALIANA (MOUSE-EAR CRESS)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27013.48

構造登録者

Sanchez-Barrena, M.J.,Martinez-Ripoll, M.,Zhu, J.K.,Albert, A. (登録日: 2004-04-15, 公開日: 2005-01-19, 最終更新日: 2024-05-08)

主引用文献

Sanchez-Barrena, M.J.,Martinez-Ripoll, M.,Zhu, J.K.,Albert, A.
The Structure of the Arabidopsis Thaliana SOS3: Molecular Mechanism of Sensing Calcium for Salt Stress Response
J.Mol.Biol., 345:1253-, 2005

PubMed Abstract: The Arabidopsis thaliana SOS3 gene encodes a calcium sensor that is required for plant salt tolerance. The SOS3 protein binds to and activates the self-inhibited SOS2 protein kinase, which mediates the expression and activities of various transporters important for ion homeostasis under salt stress. SOS3 belongs to a unique family of calcium-binding proteins that contain two pairs of EF hand motifs with four putative metal-binding sites. We report the crystal structure of a dimeric SOS3 protein in complex with calcium, and with calcium and manganese. Analytical ultracentrifugation experiments and circular dichroism measurements show that calcium binding is responsible for the dimerization of SOS3. This leads to a change in the global shape and surface properties of the protein that may be sufficient to transmit the Ca(2+) signal elicited during salt stress.

PubMed: 15644219
DOI: 10.1016/J.JMB.2004.11.025

実験手法

X-RAY DIFFRACTION (2.7 Å)