1UZZ

Erythrina cristagalli bound to N-linked oligosaccharide and lactose

1UZZ の概要

• エントリーDOI: 10.2210/pdb1uzz/pdb
• 関連するPDBエントリー: 1UZY 1V00
• 分子名称: Lectin, GLYCEROL, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: lectin, carbohydrate binding
• 由来する生物種: Erythrina crista-galli (Cockspur coral tree)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 106922.45

構造登録者

Turton, K.,Natesh, R.,Thiyagarajan, N.,Chaddock, J.A.,Acharya, K.R. (登録日: 2004-03-20, 公開日: 2004-06-22, 最終更新日: 2024-05-08)

主引用文献

Turton, K.,Natesh, R.,Thiyagarajan, N.,Chaddock, J.A.,Acharya, K.R.
Crystal structures of Erythrina cristagalli lectin with bound N-linked oligosaccharide and lactose.
Glycobiology, 14:923-929, 2004

PubMed Abstract: Erythrina cristagalli lectin (ECL) is a galactose-specific legume lectin. Although its biological function in the legume is unknown, ECL exhibits hemagglutinating activity in vitro and is mitogenic for T lymphocytes. In addition, it has been recently shown that ECL forms a novel conjugate when coupled to a catalytically active derivative of the type A neurotoxin from Clostridium botulinum, thus providing a therapeutic potential. ECL is biologically active as a dimer in which each protomer contains a functional carbohydrate-combining site. The crystal structure of native ECL was recently reported in complex with lactose and 2'-fucosyllactose. ECL protomers adopt the legume lectin fold but form non-canonical dimers via the handshake motif as was previously observed for Erythrina corallodendron lectin. Here we report the crystal structures of native and recombinant forms of the lectin in three new crystal forms, both unliganded and in complex with lactose. For the first time, the detailed structure of the glycosylated hexasaccharide for native ECL has been elucidated. The structure also shows that in the crystal lattice the glycosylation site and the carbohydrate binding site are involved in intermolecular contacts through water-mediated interactions.

PubMed: 15201215
DOI: 10.1093/glycob/cwh114

実験手法

X-RAY DIFFRACTION (2.13 Å)