1UZX

A complex of the Vps23 UEV with ubiquitin

1UZX の概要

• エントリーDOI: 10.2210/pdb1uzx/pdb
• 関連するPDBエントリー: 1AAR 1C3T 1CMX 1D3Z 1F9J 1FXT 1G6J 1GJZ 1NBF 1OGW 1P3Q 1TBE 1UBI 1UBQ 1UD7
• 分子名称: VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS23, UBIQUITIN, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (5 entities in total)
• 機能のキーワード: transport protein, transport protein-complex, uev, e2 variant, ubquitin, escrt-i, vps23, mvb sorting, nuclear protein, polyprotein, transport; protein transport; ubl conjugation pathway
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Cytoplasm: P25604
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28207.24

構造登録者

Teo, H.,Williams, R.L. (登録日: 2004-03-18, 公開日: 2004-03-30, 最終更新日: 2025-04-09)

主引用文献

Teo, H.,Veprintsev, D.,Williams, R.L.
Structural Insights Into Endosomal Sorting Complex Required for Transport (Escrt-I) Recognition of Ubiquitinated Proteins
J.Biol.Chem., 279:28689-, 2004

PubMed Abstract: The endosomal sorting complex required for transport (ESCRT-I) is a 350-kDa complex of three proteins, Vps23, Vps28, and Vps37. The N-terminal ubiquitin-conjugating enzyme E2 variant (UEV) domain of Vps23 is required for sorting ubiquitinated proteins into the internal vesicles of multivesicular bodies. UEVs are homologous to E2 ubiquitin ligases but lack the conserved cysteine residue required for catalytic activity. The crystal structure of the yeast Vps23 UEV in a complex with ubiquitin (Ub) shows the detailed interactions made with the bound Ub. Compared with the solution structure of the Tsg101 UEV (the human homologue of Vps23) in the absence of Ub, two loops that are conserved among the ESCRT-I UEVs move toward each other to grip the Ub in a pincer-like grasp. The contacts with the UEV encompass two adjacent patches on the surface of the Ub, one containing several hydrophobic residues, including Ile-8(Ub), Ile-44(Ub), and Val-70(Ub), and the second containing a hydrophilic patch including residues Asn-60(Ub), Gln-62(Ub), Glu-64(Ub). The hydrophobic Ub patch interacting with the Vps23 UEV overlaps the surface of Ub interacting with the Vps27 ubiquitin-interacting motif, suggesting a sequential model for ubiquitinated cargo binding by these proteins. In contrast, the hydrophilic patch encompasses residues uniquely interacting with the ESCRT-I UEV. The structure provides a detailed framework for design of mutants that can specifically affect ESCRT-I-dependent sorting of ubiquitinated cargo without affecting Vps27-mediated delivery of cargo to endosomes.

PubMed: 15044434
DOI: 10.1074/JBC.M400023200

実験手法

X-RAY DIFFRACTION (1.85 Å)