1UZD

Chlamydomonas,Spinach Chimeric Rubisco

Summary for 1UZD

• Entry DOI: 10.2210/pdb1uzd/pdb
• Related: 1AA1 1AUS 1GK8 1IR1 1IR2 1UPM 1UPP 1UW9 1UWA 1UZH
• Descriptor: Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain 1, chloroplastic,Ribulose bisphosphate carboxylase small chain 2, chloroplastic,Ribulose bisphosphate carboxylase small chain 1, chloroplastic, MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: lyase, rubisco, photosynthesis, carbon dioxide fixation, photorespiration, oxidoreductase, monooxygenase, chloroplast, transit peptide, multigene family
• Biological source: Chlamydomonas reinhardtii; More
• Total number of polymer chains: 16
• Total formula weight: 553738.46

Authors

Karkehabadi, S.,Spreitzer, R.J.,Andersson, I. (deposition date: 2004-03-11, release date: 2005-05-31, Last modification date: 2023-12-13)

Primary citation

Karkehabadi, S.,Peddi, S.R.,Anwaruzzaman, M.,Taylor, T.C.,Cederlund, A.,Genkov, T.,Andersson, I.,Spreitzer, R.J.
Chimeric Small Subunits Influence Catalysis without Causing Global Conformational Changes in the Crystal Structure of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
Biochemistry, 44:9851-, 2005

PubMed Abstract: Comparison of subunit sequences and X-ray crystal structures of ribulose-1,5-bisphosphate carboxylase/oxygenase indicates that the loop between beta-strands A and B of the small subunit is one of the most variable regions of the holoenzyme. In prokaryotes and nongreen algae, the loop contains 10 residues. In land plants and green algae, the loop is comprised of approximately 22 and 28 residues, respectively. Previous studies indicated that the longer betaA-betaB loop was required for the assembly of cyanobacterial small subunits with plant large subunits in isolated chloroplasts. In the present study, chimeric small subunits were constructed by replacing the loop of the green alga Chlamydomonas reinhardtii with the sequences of Synechococcus or spinach. When these engineered genes were transformed into a Chlamydomonas mutant that lacks small-subunit genes, photosynthesis-competent colonies were recovered, indicating that loop size is not essential for holoenzyme assembly. Whereas the Synechococcus loop causes decreases in carboxylation V(max), K(m)(O(2)), and CO(2)/O(2) specificity, the spinach loop causes complementary decreases in carboxylation V(max), K(m)(O(2)), and K(m)(CO(2)) without a change in specificity. X-ray crystal structures of the engineered proteins reveal remarkable similarity between the introduced betaA-betaB loops and the respective loops in the Synechococcus and spinach enzymes. The side chains of several large-subunit residues are altered in regions previously shown by directed mutagenesis to influence CO(2)/O(2) specificity. Differences in the catalytic properties of divergent Rubisco enzymes may arise from differences in the small-subunit betaA-betaB loop. This loop may be a worthwhile target for genetic engineering aimed at improving photosynthetic CO(2) fixation.

PubMed: 16026157
DOI: 10.1021/BI050537V

Experimental method

X-RAY DIFFRACTION (2.4 Å)