1UW1

A Novel ADP- and Zinc-binding fold from function-directed in vitro evolution

1UW1 の概要

• エントリーDOI: 10.2210/pdb1uw1/pdb
• 分子名称: ARTIFICIAL NUCLEOTIDE BINDING PROTEIN (ANBP), ADENOSINE-5'-DIPHOSPHATE, ZINC ION, ... (4 entities in total)
• 機能のキーワード: artificial nucleotide binding protein, nucleotide binding protein, in vitro evolution, de novo protein
• 由来する生物種: SYNTHETIC CONSTRUCT
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10098.59

構造登録者

Lo Surdo, P.,Walsh, M.A.,Sollazzo, M. (登録日: 2004-01-28, 公開日: 2004-03-26, 最終更新日: 2024-05-08)

主引用文献

Lo Surdo, P.,Walsh, M.A.,Sollazzo, M.
A Novel Adp- and Zinc-Binding Fold from Function-Directed in Vitro Evolution
Nat.Struct.Mol.Biol., 11:382-, 2004

PubMed Abstract: A great challenge to biologists is to create proteins with novel folds and tailored functions. As an alternative to de novo protein design, we investigated the structure of a randomly generated protein targeted to bind ATP. The crystal structure reveals a novel alpha/beta fold bound to its ligand, representing both the first protein structure derived from in vitro evolution and the first nucleotide-binding protein stabilized by a zinc ion.

PubMed: 15024384
DOI: 10.1038/NSMB745

実験手法

X-RAY DIFFRACTION (1.94 Å)