1UUP

Crystal Structure of A Dimeric Form of Streptococcal Pyrogenic Exotoxin A (SpeA1).

1UUP の概要

• エントリーDOI: 10.2210/pdb1uup/pdb
• 関連するPDBエントリー: 1B1Z 1FNU 1FNV 1FNW 1HA5 1L0X 1L0Y
• 分子名称: EXOTOXIN TYPE A, ZINC ION (3 entities in total)
• 機能のキーワード: toxin, molecular recognition, superantigen, exotoxin, zinc binding, dimer
• 由来する生物種: STREPTOCOCCUS PYOGENES
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 103541.62

構造登録者

Baker, M.D.,Gendlina, I.,Collins, C.M.,Acharya, K.R. (登録日: 2004-01-08, 公開日: 2004-08-12, 最終更新日: 2024-11-06)

主引用文献

Baker, M.D.,Gendlina, I.,Collins, C.M.,Acharya, K.R.
Crystal Structure of a Dimeric Form of Streptococcal Pyrogenic Exotoxin a (Spea1)
Protein Sci., 13:2285-, 2004

PubMed Abstract: Streptococcal pyrogenic exotoxin A (SpeA1) is a bacterial superantigen associated with scarlet fever and streptococcal toxic shock syndrome (STSS). SpeA1 is found in both monomeric and dimeric forms, and previous work suggested that the dimer results from an intermolecular disulfide bond between the cysteines at positions 90 of each monomer. Here, we present the crystal structure of the dimeric form of SpeA1. The toxin crystallizes in the orthorhombic space group P212121, with two dimers in the crystallographic asymmetric unit. The final structure has a crystallographic R-factor of 21.52% for 7248 protein atoms, 136 water molecules, and 4 zinc atoms (one zinc atom per molecule). The implications of SpeA1 dimer on MHC class II and T-cell receptor recognition are discussed.

PubMed: 15295110
DOI: 10.1110/PS.04826804

実験手法

X-RAY DIFFRACTION (2.6 Å)