1UTY
Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)
Summary for 1UTY
| Entry DOI | 10.2210/pdb1uty/pdb |
| Descriptor | NON-STRUCTURAL PROTEIN 2 (2 entities in total) |
| Functional Keywords | viral protein, rna binding protein |
| Biological source | BLUETONGUE VIRUS |
| Total number of polymer chains | 2 |
| Total formula weight | 42723.25 |
| Authors | Butan, C.,Van Der zandt, H.,Tucker, P. (deposition date: 2003-12-12, release date: 2004-07-13, Last modification date: 2024-05-08) |
| Primary citation | Butan, C.,Van Der Zandt, H.,Tucker, P. Structure and Assembly of the RNA Binding Domain of Bluetongue Virus Non-Structural Protein 2 J.Biol.Chem., 279:37613-, 2004 Cited by PubMed Abstract: Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex. PubMed: 15155766DOI: 10.1074/JBC.M400502200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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