1UTY

Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)

1UTY の概要

• エントリーDOI: 10.2210/pdb1uty/pdb
• 分子名称: NON-STRUCTURAL PROTEIN 2 (2 entities in total)
• 機能のキーワード: viral protein, rna binding protein
• 由来する生物種: BLUETONGUE VIRUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42723.25

構造登録者

Butan, C.,Van Der zandt, H.,Tucker, P. (登録日: 2003-12-12, 公開日: 2004-07-13, 最終更新日: 2024-05-08)

主引用文献

Butan, C.,Van Der Zandt, H.,Tucker, P.
Structure and Assembly of the RNA Binding Domain of Bluetongue Virus Non-Structural Protein 2
J.Biol.Chem., 279:37613-, 2004

PubMed Abstract: Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex.

PubMed: 15155766
DOI: 10.1074/JBC.M400502200

実験手法

X-RAY DIFFRACTION (2.4 Å)