1USR

Newcastle disease virus hemagglutinin-neuraminidase: Evidence for a second sialic acid binding site and implications for fusion

1USR の概要

• エントリーDOI: 10.2210/pdb1usr/pdb
• 関連するPDBエントリー: 1USX
• 分子名称: HEMAGGLUTININ-NEURAMINIDASE GLYCOPROTEIN, N-acetyl-alpha-neuraminic acid-(2-6)-methyl 6-thio-beta-D-galactopyranoside, 2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC ACID, ... (7 entities in total)
• 機能のキーワード: hydrolase, sialidase, neuraminidase, hemagglutinin-neuraminidase, hemagglutinin, glycoprotein
• 由来する生物種: NEWCASTLE DISEASE VIRUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 101629.68

構造登録者

Zaitsev, V.,Von Itzstein, M.,Groves, D.,Kiefel, M.,Takimoto, T.,Portner, A.,Taylor, G. (登録日: 2003-11-28, 公開日: 2004-01-02, 最終更新日: 2024-11-20)

主引用文献

Zaitsev, V.,Von Itzstein, M.,Groves, D.,Kiefel, M.,Takimoto, T.,Portner, A.,Taylor, G.
Second Sialic Acid Binding Site in Newcastle Disease Virus Hemagglutinin-Neuraminidase: Implications for Fusion
J.Virol., 78:3733-, 2004

PubMed Abstract: Paramyxoviruses are the leading cause of respiratory disease in children. Several paramyxoviruses possess a surface glycoprotein, the hemagglutinin-neuraminidase (HN), that is involved in attachment to sialic acid receptors, promotion of fusion, and removal of sialic acid from infected cells and progeny virions. Previously we showed that Newcastle disease virus (NDV) HN contained a pliable sialic acid recognition site that could take two states, a binding state and a catalytic state. Here we present evidence for a second sialic acid binding site at the dimer interface of HN and present a model for its involvement in cell fusion. Three different crystal forms of NDV HN now reveal identical tetrameric arrangements of HN monomers, perhaps indicative of the tetramer association found on the viral surface.

PubMed: 15016893
DOI: 10.1128/JVI.78.7.3733-3741.2004

実験手法

X-RAY DIFFRACTION (2 Å)