1URY

cytoglobin cavities

1URY の概要

• エントリーDOI: 10.2210/pdb1ury/pdb
• 関連するPDBエントリー: 1UMO 1URV
• 分子名称: CYTOGLOBIN, PROTOPORPHYRIN IX CONTAINING FE, XENON, ... (5 entities in total)
• 機能のキーワード: globin, cytoglobin, histoglobin, heme, hexa-coordination, protein, oxygen transport
• 由来する生物種: HOMO SAPIENS
• 細胞内の位置: Cytoplasm (By similarity): Q8WWM9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45378.89

構造登録者

de Sanctis, D.,Dewilde, S.,Pesce, A.,Moens, L.,Ascenzi, P.,Hankeln, T.,Burmester, T.,Bolognesi, M. (登録日: 2003-11-12, 公開日: 2004-12-15, 最終更新日: 2024-05-08)

主引用文献

de Sanctis, D.,Dewilde, S.,Pesce, A.,Moens, L.,Ascenzi, P.,Hankeln, T.,Burmester, T.,Bolognesi, M.
Cytoglobin Cavities
Biochem.Biophys.Res.Commun., 316:1217-, 2004

PubMed Abstract: Cytoglobin is the fourth recognized globin type, almost ubiquitously distributed in human tissues; its function is still poorly understood. Cytoglobin displays a core region of about 150 residues, structurally related to hemoglobin and myoglobin, and two extra segments, about 20 residues each, at the N- and C-termini. The core region hosts a large apolar cavity, held to provide a ligand diffusion pathway to/from the heme, and/or ligand temporary docking sites. Here we report the crystal structure (2.4A resolution, R-factor 19.1%) of a human cytoglobin mutant bearing the CysB2(38) --> Ser and CysE9(83) --> Ser substitutions (CYGB*), treated under pressurized xenon. Three Xe atoms bind to the heme distal site region of CYGB* mapping the protein matrix apolar cavity. Despite the conserved globin fold, the cavity found in CYGB* is structured differently from those recognized to play a functional role in myoglobin, neuroglobin, truncated hemoglobins, and Cerebratulus lacteus mini-hemoglobin.

PubMed: 15044115
DOI: 10.1016/J.BBRC.2004.03.007

実験手法

X-RAY DIFFRACTION (2.4 Å)