1URJ

Single stranded DNA-binding protein(ICP8) from Herpes simplex virus-1

1URJ の概要

• エントリーDOI: 10.2210/pdb1urj/pdb
• 分子名称: MAJOR DNA-BINDING PROTEIN, ZINC ION, MERCURY (II) ION, ... (4 entities in total)
• 機能のキーワード: dna-binding protein, ssb, icp8, hsv-1, dna-binding, dna replication, zinc-finger, nuclear protein., dna binding protein
• 由来する生物種: HUMAN HERPESVIRUS 1 (HERPES VIRUS)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 246271.13

構造登録者

Panjikar, S.,Mapelli, M.,Tucker, P.A. (登録日: 2003-10-30, 公開日: 2004-11-11, 最終更新日: 2024-05-08)

主引用文献

Mapelli, M.,Panjikar, S.,Tucker, P.A.
The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding.
J. Biol. Chem., 280:2990-2997, 2005

PubMed Abstract: All organisms including animal viruses use specific proteins to bind single-stranded DNA rapidly in a non-sequence-specific, flexible, and cooperative manner during the DNA replication process. The crystal structure of a 60-residue C-terminal deletion construct of ICP8, the major single-stranded DNA-binding protein from herpes simplex virus-1, was determined at 3.0 A resolution. The structure reveals a novel fold, consisting of a large N-terminal domain (residues 9-1038) and a small C-terminal domain (residues 1049-1129). On the basis of the structure and the nearest neighbor interactions in the crystal, we have presented a model describing the site of single-stranded DNA binding and explaining the basis for cooperative binding. This model agrees with the beaded morphology observed in electron micrographs.

PubMed: 15507432
DOI: 10.1074/jbc.M406780200

実験手法

X-RAY DIFFRACTION (3 Å)