1UP7

Structure of the 6-phospho-beta glucosidase from Thermotoga maritima at 2.4 Angstrom resolution in the tetragonal form with NAD and glucose-6-phosphate

1UP7 の概要

• エントリーDOI: 10.2210/pdb1up7/pdb
• 関連するPDBエントリー: 1UP4 1UP6
• 分子名称: 6-PHOSPHO-BETA-GLUCOSIDASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, 6-O-phosphono-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: hydrolase, 6-phospho-beta-glucosidase, family4 hydrolase, nad dependent
• 由来する生物種: THERMOTOGA MARITIMA
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 391493.02

構造登録者

Varrot, A.,Yip, V.L.,Withers, S.G.,Davies, G.J. (登録日: 2003-09-29, 公開日: 2004-11-18, 最終更新日: 2024-05-01)

主引用文献

Varrot, A.,Yip, V.L.,Li, Y.,Rajan, S.S.,Yang, X.,Anderson, W.F.,Thompson, J.,Withers, S.G.,Davies, G.J.
Nad+ and Metal-Ion Dependent Hydrolysis by Family 4 Glycosidases: Structural Insight Into Specificity for Phospho-Beta-D-Glucosides
J.Mol.Biol., 346:423-, 2005

PubMed Abstract: The import of disaccharides by many bacteria is achieved through their simultaneous translocation and phosphorylation by the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). The imported phospho-disaccharides are, in some cases, subsequently hydrolyzed by members of the unusual glycoside hydrolase family GH4. The GH4 enzymes, occasionally found also in bacteria such as Thermotoga maritima that do not utilise a PEP-PTS system, require both NAD(+) and Mn(2+) for catalysis. A further curiosity of this family is that closely related enzymes may show specificity for either alpha-d- or beta-d-glycosides. Here, we present, for the first time, the three-dimensional structure (using single-wavelength anomalous dispersion methods, harnessing extensive non-crystallographic symmetry) of the 6-phospho-beta-glycosidase, BglT, from T.maritima in native and complexed (NAD(+) and Glc6P) forms. Comparison of the active-center structure with that of the 6-phospho-alpha-glucosidase GlvA from Bacillus subtilis reveals a striking degree of structural similarity that, in light of previous kinetic isotope effect data, allows the postulation of a common reaction mechanism for both alpha and beta-glycosidases. Given that the "chemistry" occurs primarily on the glycone sugar and features no nucleophilic attack on the intact disaccharide substrate, modulation of anomeric specificity for alpha and beta-linkages is accommodated through comparatively minor structural changes.

PubMed: 15670594
DOI: 10.1016/J.JMB.2004.11.058

実験手法

X-RAY DIFFRACTION (2.4 Å)