1UOZ

Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with thiocellopentaose at 1.1 angstrom

Summary for 1UOZ

• Entry DOI: 10.2210/pdb1uoz/pdb
• Related: 1UP0 1UP2 1UP3 1UP4 1UP6 1UP7
• Descriptor: PUTATIVE CELLULASE, alpha-D-glucopyranose-(1-4)-1,4-dithio-beta-D-glucopyranose-(1-4)-1,4-dithio-beta-D-glucopyranose, 4-thio-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: hydrolase, glycoside hydrolase, family 6
• Biological source: MYCOBACTERIUM TUBERCULOSIS
• Total number of polymer chains: 1
• Total formula weight: 34129.56

Authors

Varrot, A.,Leydier, S.,Pell, G.,Gilbert, H.J.,Davies, G.J. (deposition date: 2003-09-26, release date: 2004-11-18, Last modification date: 2024-10-16)

Primary citation

Varrot, A.,Leydier, S.,Pell, G.,Macdonald, J.M.,Stick, R.V.,Henrissat, B.,Gilbert, H.J.,Davies, G.J.
Mycobacterium Tuberculosis Strains Possess Functional Cellulases.
J.Biol.Chem., 280:20181-, 2005

PubMed Abstract: The genomes of various Mycobacterium tuberculosis strains encode proteins that do not appear to play a role in the growth or survival of the bacterium in its mammalian host, including some implicated in plant cell wall breakdown. Here we show that M. tuberculosis H37Rv does indeed possess a functional cellulase. The x-ray crystal structure of this enzyme, in ligand complex forms, from 1.9 to 1.1A resolution, reveals a highly conserved substrate-binding cleft, which affords similar, and unusual, distortion of the substrate at the catalytic center. The endoglucanase activity, together with the existence of a putative membrane-associated crystalline polysaccharide-binding protein, may reflect the ancestral soil origin of the Mycobacterium or hint at a previously unconsidered environmental niche.

PubMed: 15824123
DOI: 10.1074/JBC.C500142200

Experimental method

X-RAY DIFFRACTION (1.1 Å)