1UMI

Structural basis of sugar-recognizing ubiquitin ligase

1UMI の概要

• エントリーDOI: 10.2210/pdb1umi/pdb
• 関連するPDBエントリー: 1UMH
• 分子名称: F-box only protein 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose (3 entities in total)
• 機能のキーワード: ubiquitin, scf, ubiquitin ligase, lectin, ligase
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21413.35

構造登録者

Mizushima, T.,Hirao, T.,Yoshida, Y.,Lee, S.J.,Chiba, T.,Iwai, K.,Yamaguchi, Y.,Kato, K.,Tsukihara, T.,Tanaka, K. (登録日: 2003-10-01, 公開日: 2004-04-06, 最終更新日: 2023-12-27)

主引用文献

Mizushima, T.,Hirao, T.,Yoshida, Y.,Lee, S.J.,Chiba, T.,Iwai, K.,Yamaguchi, Y.,Kato, K.,Tsukihara, T.,Tanaka, K.
Structural basis of sugar-recognizing ubiquitin ligase.
Nat.Struct.Mol.Biol., 11:365-370, 2004

PubMed Abstract: SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic reticulum (ER)-associated degradation pathway. Fbs1/Fbx2, a member of the F-box proteins, recognizes high-mannose oligosaccharides. Efficient binding to an N-glycan requires di-N-acetylchitobiose (chitobiose). Here we report the crystal structures of the sugar-binding domain (SBD) of Fbs1 alone and in complex with chitobiose. The SBD is composed of a ten-stranded antiparallel beta-sandwich. The structure of the SBD-chitobiose complex includes hydrogen bonds between Fbs1 and chitobiose and insertion of the methyl group of chitobiose into a small hydrophobic pocket of Fbs1. Moreover, NMR spectroscopy has demonstrated that the amino acid residues adjoining the chitobiose-binding site interact with the outer branches of the carbohydrate moiety. Considering that the innermost chitobiose moieties in N-glycans are usually involved in intramolecular interactions with the polypeptide moieties, we propose that Fbs1 interacts with the chitobiose in unfolded N-glycoprotein, pointing the protein moiety toward E2 for ubiquitination.

PubMed: 14990996
DOI: 10.1038/nsmb732

実験手法

X-RAY DIFFRACTION (2.4 Å)