1UHN

The crystal structure of the calcium binding protein AtCBL2 from Arabidopsis thaliana

1UHN の概要

• エントリーDOI: 10.2210/pdb1uhn/pdb
• 関連するPDBエントリー: 1AUI
• 分子名称: calcineurin B-like protein 2, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium binding protein, metal binding protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21988.05

構造登録者

Nagae, M.,Nozawa, A.,Koizumi, N.,Sano, H.,Hashimoto, H.,Sato, M.,Shimizu, T. (登録日: 2003-07-07, 公開日: 2003-11-04, 最終更新日: 2023-12-27)

主引用文献

Nagae, M.,Nozawa, A.,Koizumi, N.,Sano, H.,Hashimoto, H.,Sato, M.,Shimizu, T.
The Crystal Structure of the Novel Calcium-binding Protein AtCBL2 from Arabidopsis thaliana
J.Biol.Chem., 278:42240-42246, 2003

PubMed Abstract: Arabidopsis thaliana calcineurin B-like protein (AtCBL2) is a member of a recently identified family of calcineurin B-like calcium-binding proteins in A. thaliana. The crystal structure of AtCBL2 has been determined at 2.1 A resolution. The protein forms a compact alpha-helical structure with two pairs of EF-hand motifs. The structure is similar in overall folding topology to the structures of calcineurin B and neuronal calcium sensor 1, but differs significantly in local conformation. The two calcium ions are coordinated in the first and fourth EF-hand motifs, whereas the second and third EF-hand motifs are maintained in the open form by internal hydrogen bonding without coordination of calcium ions. Both a possible site and a possible mechanism for the target binding to AtCBL2 are discussed based on the three-dimensional structure.

PubMed: 12871972
DOI: 10.1074/jbc.M303630200

実験手法

X-RAY DIFFRACTION (2.1 Å)