1UHA

Crystal Structure of Pokeweed Lectin-D2

1UHA の概要

• エントリーDOI: 10.2210/pdb1uha/pdb
• 分子名称: lectin-D2, CALCIUM ION (3 entities in total)
• 機能のキーワード: lectin, chitin-binding domain, sugar binding protein
• 由来する生物種: Phytolacca americana (American pokeweed)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9152.04

構造登録者

Fujii, T.,Hayashida, M.,Hamasu, M.,Ishiguro, M.,Hata, Y. (登録日: 2003-06-27, 公開日: 2004-04-13, 最終更新日: 2024-10-16)

主引用文献

Fujii, T.,Hayashida, M.,Hamasu, M.,Ishiguro, M.,Hata, Y.
Structures of two lectins from the roots of pokeweed (Phytolacca americana).
Acta Crystallogr.,Sect.D, 60:665-673, 2004

PubMed Abstract: Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous domains but to have different molecular sizes and biological properties. PL-D, the smallest lectin in the group, has two isolectins, PL-D1 and PL-D2. PL-D1 consists of 84 amino-acid residues, while PL-D2 is identical to PL-D1 in sequence except for the lack of two C-terminal residues, Leu83 and Thr84. The crystal structures of PL-D1 and PL-D2 were solved by the molecular-replacement method and refined to 1.65 and 1.5 A resolution with R factors of 17.2 and 17.6%, respectively. The PL-Ds are composed of two repetitive chitin-binding domains, each of which has four S-S bridges and one putative carbohydrate-binding site. The two carbohydrate-binding sites in PL-D are located on one side of the molecule. The relative orientation of the two domains in PL-D1 differs from that in PL-D2. Two C-terminal residues of PL-D1 are invisible in the present crystal structure, indicating the flexibility of the region. PL-D2 has a Ca2+ ion bound to the C-terminus on the molecular surface. A wide distribution of acidic residues is characteristically observed on one side of the C-terminal region of PL-D.

PubMed: 15039554
DOI: 10.1107/S090744490400232X

実験手法

X-RAY DIFFRACTION (1.5 Å)