1UEJ

Crystal structure of human uridine-cytidine kinase 2 complexed with a substrate, cytidine

1UEJ の概要

• エントリーDOI: 10.2210/pdb1uej/pdb
• 関連するPDBエントリー: 1UDW 1UEI
• 分子名称: Uridine-cytidine kinase 2, 4-AMINO-1-BETA-D-RIBOFURANOSYL-2(1H)-PYRIMIDINONE, CITRIC ACID, ... (4 entities in total)
• 機能のキーワード: alpha/beta mononucleotide-binding hold, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57396.67

構造登録者

Suzuki, N.N.,Koizumi, K.,Fukushima, M.,Matsuda, A.,Inagaki, F. (登録日: 2003-05-16, 公開日: 2004-05-04, 最終更新日: 2023-10-25)

主引用文献

Suzuki, N.N.,Koizumi, K.,Fukushima, M.,Matsuda, A.,Inagaki, F.
Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase
STRUCTURE, 12:751-764, 2004

PubMed Abstract: Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and cytidine and activates pharmacological ribonucleoside analogs. Here we present the crystal structures of human UCK alone and in complexes with a substrate, cytidine, a feedback inhibitor, CTP or UTP, and with phosphorylation products, CMP and ADP, respectively. Free UCK takes an alpha/beta mononucleotide binding fold and exists as a homotetramer with 222 symmetry. Upon inhibitor binding, one loop region was loosened, causing the UCK tetramer to be distorted. Upon cytidine binding, a large induced fit was observed at the uridine/cytidine binding site, which endows UCK with a strict specificity for pyrimidine ribonucleosides. The first UCK structure provided the structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase, which give clues for the design of novel antitumor and antiviral ribonucleoside analogs that inhibit RNA synthesis.

PubMed: 15130468
DOI: 10.1016/j.str.2004.02.038

実験手法

X-RAY DIFFRACTION (2.61 Å)