1UCU

R-type straight flagellar filament made of full-length flagellin

1UCU の概要

• エントリーDOI: 10.2210/pdb1ucu/pdb
• 分子名称: phase 1 Flagellin (1 entity in total)
• 機能のキーワード: flagellin, flagellar filament, helical reconstruction, structural protein
• 由来する生物種: Salmonella typhimurium
• 細胞内の位置: Secreted: P06179
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51551.20

構造登録者

Yonekura, K.,Maki-Yonekura, S.,Namba, K. (登録日: 2003-04-22, 公開日: 2003-08-12, 最終更新日: 2024-03-13)

主引用文献

Yonekura, K.,Maki-Yonekura, S.,Namba, K.
Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy
NATURE, 424:643-650, 2003

PubMed Abstract: The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagellin fragment lacking about 100 terminal residues revealed the protofilament structure, but the full filament structure is still essential for understanding the mechanism of supercoiling and polymerization. Here we report a complete atomic model of the R-type filament by electron cryomicroscopy. A density map obtained from image data up to 4 A resolution shows the feature of alpha-helical backbone and some large side chains. The atomic model built on the map reveals intricate molecular packing and an alpha-helical coiled coil formed by the terminal chains in the inner core of the filament, with its intersubunit hydrophobic interactions having an important role in stabilizing the filament.

PubMed: 12904785
DOI: 10.1038/nature01830

実験手法

ELECTRON MICROSCOPY (4 Å)