1UC6

Solution Structure of the Carboxyl Terminal Domain of the Ciliary Neurotrophic Factor Receptor

1UC6 の概要

• エントリーDOI: 10.2210/pdb1uc6/pdb
• 分子名称: Ciliary Neurotrophic Factor Receptor alpha (1 entity in total)
• 機能のキーワード: cytokine, ciliary neurotrophic factor, leukemia inhibitory factor, cytokine receptor, fibronectin type iii domain-like topology, seven beta-strands, two anti-parallel beta-sheets, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Lipid-anchor, GPI-anchor: P26992
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12333.68

構造登録者

Man, D.,He, W.,Sze, K.H.,Ke, G.,Smith, D.K.,Ip, N.Y.,Zhu, G. (登録日: 2003-04-08, 公開日: 2004-08-10, 最終更新日: 2023-12-27)

主引用文献

Man, D.,He, W.,Sze, K.H.,Gong, K.,Smith, D.K.,Zhu, G.,Ip, N.Y.
Solution structure of the C-terminal domain of the ciliary neurotrophic factor (CNTF) receptor and ligand free associations among components of the CNTF receptor complex
J.Biol.Chem., 278:23285-23294, 2003

PubMed Abstract: The functional receptor complex of ciliary neurotrophic factor (CNTF), a member of the gp130 family of cytokines, is composed of CNTF, the CNTF receptor alpha (CNTFR), gp130, and the leukemia inhibitory factor receptor (LIFR). However, the nature of the receptor-mediated interactions in this complex has not yet been resolved. To address this issue we have determined the solution structure of the C-terminal or BC domain of CNTFR and studied the interactions of CNTFR with LIFR and gp130. We reported previously that the membrane distal cytokine-binding domain (CBD1) of LIFR could interact in vitro with soluble CNTFR (sCNTFR) in the absence of CNTF. Here we show that the CBD of human gp130 can also bind in vitro to sCNTFR in the absence of CNTF. In addition, the gp130 CBD could compete with the LIFR CBD1 for the binding of sCNTFR. Substitution of residues in the gp130 CBD, the LIFR CBD1, and the CNTFR BC domain that are expected to be involved in receptor-receptor interactions significantly reduced their interactions. An NMR chemical shift perturbation study of the interaction between the BC domains of CNTFR and gp130 further mapped the interaction surface. These data suggest that both the gp130 CBD and the LIFR CBD1 interact with CNTFR in a similar way and provide insights into the nature of the CNTF receptor complex.

PubMed: 12707266
DOI: 10.1074/jbc.M301976200

実験手法

SOLUTION NMR