1UA7

Crystal Structure Analysis of Alpha-Amylase from Bacillus Subtilis complexed with Acarbose

Summary for 1UA7

Entry DOI	10.2210/pdb1ua7/pdb
Related	1BAG
Descriptor	Alpha-amylase, 4,6-dideoxy-alpha-D-xylo-hexopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-quinovopyranose-(1-4)-beta-D-glucopyranose, ... (6 entities in total)
Functional Keywords	beta-alpha-barrels, acarbose, greek-key motif, hydrolase
Biological source	Bacillus subtilis
Cellular location	Secreted : P00691
Total number of polymer chains	1
Total formula weight	47904.18
Authors	Kagawa, M.,Fujimoto, Z.,Momma, M.,Takase, K.,Mizuno, H. (deposition date: 2003-03-03, release date: 2004-05-18, Last modification date: 2023-12-27)
Primary citation	Kagawa, M.,Fujimoto, Z.,Momma, M.,Takase, K.,Mizuno, H. Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose J.BACTERIOL., 185:6981-6984, 2003 Cited by PubMed Abstract: The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native substrate maltopentaose, it is suggested that the present structure represents a mimic intermediate in the initial stage of the catalytic process. PubMed: 14617662 DOI: 10.1128/JB.185.23.6981-6984.2003 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.21 Å)

Structure validation