1U89

Solution structure of VBS2 fragment of talin

1U89 の概要

• エントリーDOI: 10.2210/pdb1u89/pdb
• 関連するPDBエントリー: 1SJ7 1SJ8
• NMR情報: BMRB: 6285
• 分子名称: Talin 1 (1 entity in total)
• 機能のキーワード: 4-helix bundle, left-handed, structural protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P26039
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14396.91

構造登録者

Fillingham, I.,Gingras, A.R.,Papagrigoriou, E.,Patel, B.,Emsley, J.,Roberts, G.C.K.,Critchley, D.R.,Barsukov, I.L. (登録日: 2004-08-05, 公開日: 2005-01-18, 最終更新日: 2024-05-29)

主引用文献

Fillingham, I.,Gingras, A.R.,Papagrigoriou, E.,Patel, B.,Emsley, J.,Critchley, D.R.,Roberts, G.C.,Barsukov, I.L.
A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head.
Structure, 13:65-74, 2005

PubMed Abstract: The cytoskeletal protein talin plays a key role in activating integrins and in coupling them to the actin cytoskeleton. Its N-terminal globular head, which binds beta integrins, is linked to an extended rod having a C-terminal actin binding site and several vinculin binding sites (VBSs). The NMR structure of residues 755-889 of the rod (containing a VBS) is shown to be an amphipathic four-helix bundle with a left-handed topology. A talin peptide corresponding to the VBS binds the vinculin head; the X-ray crystallographic structure of this complex shows that the residues which interact with vinculin are buried in the hydrophobic core of the talin fragment. NMR shows that the interaction involves a major structural change in the talin fragment, including unfolding of one of its helices, making the VBS accessible to vinculin. Interestingly, the talin 755-889 fragment binds more than one vinculin head molecule, suggesting that the talin rod may contain additional as yet unrecognized VBSs.

PubMed: 15642262
DOI: 10.1016/j.str.2004.11.006

実験手法

SOLUTION NMR