1U6B

CRYSTAL STRUCTURE OF A SELF-SPLICING GROUP I INTRON WITH BOTH EXONS

「1T42」から置き換えられました

1U6B の概要

• エントリーDOI: 10.2210/pdb1u6b/pdb
• 分子名称: 197-MER, 5'-R(*AP*AP*GP*CP*CP*AP*CP*AP*CP*AP*AP*AP*CP*CP*AP*GP*AP*CP*GP *GP*CP*C)-3', 5'-R(*CP*AP*(5MU))-3', ... (7 entities in total)
• 機能のキーワード: intron, exon, ribozyme, group i, u1a, rna, structural protein-rna complex, structural protein/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P09012
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 83863.77

構造登録者

Adams, P.L.,Stahley, M.R.,Kosek, A.B.,Wang, J.,Strobel, S.A. (登録日: 2004-07-29, 公開日: 2004-08-10, 最終更新日: 2024-02-14)

主引用文献

Adams, P.L.,Stahley, M.R.,Kosek, A.B.,Wang, J.,Strobel, S.A.
Crystal Structure of a Self-Splicing Group I Intron with Both Exons.
Nature, 430:45-50, 2004

PubMed Abstract: The discovery of the RNA self-splicing group I intron provided the first demonstration that not all enzymes are proteins. Here we report the X-ray crystal structure (3.1-A resolution) of a complete group I bacterial intron in complex with both the 5'- and the 3'-exons. This complex corresponds to the splicing intermediate before the exon ligation step. It reveals how the intron uses structurally unprecedented RNA motifs to select the 5'- and 3'-splice sites. The 5'-exon's 3'-OH is positioned for inline nucleophilic attack on the conformationally constrained scissile phosphate at the intron-3'-exon junction. Six phosphates from three disparate RNA strands converge to coordinate two metal ions that are asymmetrically positioned on opposing sides of the reactive phosphate. This structure represents the first splicing complex to include a complete intron, both exons and an organized active site occupied with metal ions.

PubMed: 15175762
DOI: 10.1038/nature02642

実験手法

X-RAY DIFFRACTION (3.1 Å)