1U5K

Recombinational repair protein RecO

1U5K の概要

• エントリーDOI: 10.2210/pdb1u5k/pdb
• 分子名称: hypothetical protein, ZINC ION (3 entities in total)
• 機能のキーワード: obd-fold, zn-binding, recombination, replication
• 由来する生物種: Deinococcus radiodurans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52883.70

構造登録者

Makharashvili, N.,Koroleva, O.,Bera, S.,Grandgenett, D.P.,Korolev, S. (登録日: 2004-07-27, 公開日: 2004-10-26, 最終更新日: 2024-02-14)

主引用文献

Makharashvili, N.,Koroleva, O.,Bera, S.,Grandgenett, D.P.,Korolev, S.
A Novel Structure of DNA Repair Protein RecO from Deinococcus radiodurans
STRUCTURE, 12:1881-1889, 2004

PubMed Abstract: Recovery of arrested replication requires coordinated action of DNA repair, replication, and recombination machineries. Bacterial RecO protein is a member of RecF recombination repair pathway important for replication recovery. RecO possesses two distinct activities in vitro, closely resembling those of eukaryotic protein Rad52: DNA annealing and RecA-mediated DNA recombination. Here we present the crystal structure of the RecO protein from the extremely radiation resistant bacteria Deinococcus radiodurans (DrRecO) and characterize its DNA binding and strand annealing properties. The RecO structure is totally different from the Rad52 structure. DrRecO is comprised of three structural domains: an N-terminal domain which adopts an OB-fold, a novel alpha-helical domain, and an unusual zinc-binding domain. Sequence alignments suggest that the multidomain architecture is conserved between RecO proteins from other bacterial species and is suitable to elucidate sites of protein-protein and DNA-protein interactions necessary for RecO functions during the replication recovery and DNA repair.

PubMed: 15458636
DOI: 10.1016/j.str.2004.08.006

実験手法

X-RAY DIFFRACTION (2 Å)