1U55

Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (oxygen complex)

1U55 の概要

• エントリーDOI: 10.2210/pdb1u55/pdb
• 関連するPDBエントリー: 1U4H 1U56
• 分子名称: Heme-based Methyl-accepting Chemotaxis Protein, PROTOPORPHYRIN IX CONTAINING FE, OXYGEN MOLECULE, ... (5 entities in total)
• 機能のキーワード: h-nox domain, heme, oxygen sensor, signal transduction, chemotaxis, signaling protein
• 由来する生物種: Thermoanaerobacter tengcongensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45427.43

構造登録者

Pellicena, P.,Karow, D.S.,Boon, E.M.,Marletta, M.A.,Kuriyan, J. (登録日: 2004-07-27, 公開日: 2004-08-31, 最終更新日: 2023-08-23)

主引用文献

Pellicena, P.,Karow, D.S.,Boon, E.M.,Marletta, M.A.,Kuriyan, J.
Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases.
Proc.Natl.Acad.Sci.Usa, 101:12854-12859, 2004

PubMed Abstract: Soluble guanylate cyclases are nitric oxide-responsive signaling proteins in which the nitric oxide sensor is a heme-binding domain of unknown structure that we have termed the heme-NO and oxygen binding (H-NOX) domain. H-NOX domains are also found in bacteria, either as isolated domains, or are fused through a membrane-spanning region to methyl-accepting chemotaxis proteins. We have determined the crystal structure of an oxygen-binding H-NOX domain of one such signaling protein from the obligate anaerobe Thermoanaerobacter tengcongensis at 1.77-angstroms resolution, revealing a protein fold unrelated to known structures. Particularly striking is the structure of the protoporphyrin IX group, which is distorted from planarity to an extent not seen before in protein-bound heme groups. Comparison of the structure of the H-NOX domain in two different crystal forms suggests a mechanism whereby alteration in the degree of distortion of the heme group is coupled to changes on the molecular surface of the H-NOX domain and potentially to changes in intermolecular interactions.

PubMed: 15326296
DOI: 10.1073/pnas.0405188101

実験手法

X-RAY DIFFRACTION (1.77 Å)