1U4C

Structure of spindle checkpoint protein Bub3

1U4C の概要

• エントリーDOI: 10.2210/pdb1u4c/pdb
• 分子名称: Cell cycle arrest protein BUB3 (2 entities in total)
• 機能のキーワード: wd40 protein, wd-40 protein, beta propeller, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79390.63

構造登録者

Larsen, N.A.,Harrison, S.C. (登録日: 2004-07-23, 公開日: 2004-08-03, 最終更新日: 2024-11-06)

主引用文献

Larsen, N.A.,Harrison, S.C.
Crystal structure of the spindle assembly checkpoint protein Bub3
J.Mol.Biol., 344:885-892, 2004

PubMed Abstract: Bub3 is one of at least six proteins that transmit the spindle assembly checkpoint signal. These proteins delay cell cycle progression from metaphase to anaphase in response to attachment defects between kinetochores and spindle microtubules and to tension defects between sister chromatids. To explore the molecular interactions mediated by Bub3, we have determined the crystal structure of the Saccharomyces cerevisiae protein Bub3p at 2.35 A resolution. Bub3p is a seven-blade beta-propeller, although its sequence diverges from that of other WD40 family members. Several loops are substantially elongated, but extra domains or insertions are not present at the termini. In particular, two extended loops project from the top face of the propeller, forming a cleft. Amino acid residues across the top face and one aspect of the lateral surface (spanning blades 5-6) are highly conserved among Bub3 proteins. We propose that these conserved surfaces are the loci for key interactions with conserved motifs in spindle checkpoint proteins Bub1 and Mad3/BubR1. Comparison of the Bub3 sequence to the WD40 protein, Rae1, shows high sequence conservation along the same surfaces. Rae1 interaction with Bub1 is, therefore, likely to involve a similar mode of binding.

PubMed: 15544799
DOI: 10.1016/j.jmb.2004.09.094

実験手法

X-RAY DIFFRACTION (2.35 Å)