1U0R

Crystal structure of Mycobacterium tuberculosis NAD kinase

1U0R の概要

• エントリーDOI: 10.2210/pdb1u0r/pdb
• 関連するPDBエントリー: 1U0T
• 分子名称: Inorganic polyphosphate/ATP-NAD kinase (2 entities in total)
• 機能のキーワード: alpha-beta; beta sandwich, structural genomics, psi, protein structure initiative, tb structural genomics consortium, tbsgc, signaling protein, transferase
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Cytoplasm: P0A5S6
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 131774.62

構造登録者

Garavaglia, S.,Raffaelli, N.,Finaurini, L.,Magni, G.,Rizzi, M.,TB Structural Genomics Consortium (TBSGC) (登録日: 2004-07-14, 公開日: 2004-08-31, 最終更新日: 2024-02-14)

主引用文献

Garavaglia, S.,Raffaelli, N.,Finaurini, L.,Magni, G.,Rizzi, M.
A novel fold revealed by Mycobacterium tuberculosis NAD kinase, a key allosteric enzyme in NADP biosynthesis
J.Biol.Chem., 279:40980-40986, 2004

PubMed Abstract: NAD kinase catalyzes the magnesium-dependent phosphorylation of NAD, representing the sole source of freshly synthesized NADP in all organisms. The enzyme is essential for the growth of the deadly multidrug-resistant pathogen Mycobacterium tuberculosis and is an attractive target for novel antitubercular agents. The crystal structure of NAD kinase has been solved by multiwavelength anomalous dispersion at a resolution of 2.3 A in its T state. Two crystal forms have been obtained revealing either a dimer or a tetramer. The enzyme architecture discloses a novel molecular arrangement, with each subunit consisting of an alpha/beta N-terminal domain and a C-terminal 12-stranded beta sandwich domain, connected by swapped beta strands. The C-terminal domain shows a striking internal approximate 222 symmetry and an unprecedented topology, revealing a novel fold within the family of all beta structures. The catalytic site is located in the long crevice that defines the interface between the domains. The conserved GGDG structural fingerprint of the catalytic site is reminiscent of the related region in 6-phosphofructokinase, supporting the hypothesis that NAD kinase belongs to a newly reported superfamily of kinases.

PubMed: 15269221
DOI: 10.1074/jbc.M406586200

実験手法

X-RAY DIFFRACTION (2.8 Å)