1U0I

IAAL-E3/K3 heterodimer

1U0I の概要

• エントリーDOI: 10.2210/pdb1u0i/pdb
• NMR情報: BMRB: 6260
• 分子名称: IAAL-E3, IAAL-K3 (2 entities in total)
• 機能のキーワード: coiled-coil, de novo protein
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4571.50

構造登録者

Lindhout, D.A.,Litowski, J.R.,Mercier, P.,Hodges, R.S.,Sykes, B.D. (登録日: 2004-07-13, 公開日: 2004-10-19, 最終更新日: 2024-05-22)

主引用文献

Lindhout, D.A.,Litowski, J.R.,Mercier, P.,Hodges, R.S.,Sykes, B.D.
NMR solution structure of a highly stable de novo heterodimeric coiled-coil
Biopolymers, 75:367-375, 2004

PubMed Abstract: The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic interactions at the interface crossing the hydrophobic core which direct heterodimer formation. This E3/K3 domain has previously been shown to have high alpha-helical content as well as possessing a low dissociation constant (70 nM). The E3/K3 structure is completely alpha-helical and is an archetypical coiled-coil in solution, as determined using a combination of (1)H-NOE and homology based structural restraints. This structure provides a structural framework for visualizing the important interactions for stability and specificity, which are key to protein engineering applications such as affinity purification and de novo design.

PubMed: 15457434
DOI: 10.1002/bip.20150

実験手法

SOLUTION NMR