1U0D

Y33H Mutant of Homing endonuclease I-CreI

Summary for 1U0D

• Entry DOI: 10.2210/pdb1u0d/pdb
• Related: 1U0C
• Descriptor: 5'-D(*GP*CP*GP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3', 5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*CP*GP*C)-3', DNA endonuclease I-CreI (3 entities in total)
• Functional Keywords: dna endonuclease i-crei, protein/dna, hydrolase-dna complex, hydrolase/dna
• Biological source: Chlamydomonas reinhardtii
• Cellular location: Plastid, chloroplast: P05725
• Total number of polymer chains: 4
• Total formula weight: 52138.54

Authors

Sussman, D.,Chadsey, M.,Fauce, S.,Engel, A.,Bruett, A.,Monnat, R.,Stoddard, B.L.,Seligman, L.M. (deposition date: 2004-07-13, release date: 2004-11-02, Last modification date: 2024-02-14)

Primary citation

Sussman, D.,Chadsey, M.,Fauce, S.,Engel, A.,Bruett, A.,Monnat, R.,Stoddard, B.L.,Seligman, L.M.
Isolation and characterization of new homing endonuclease specificities at individual target site positions.
J.Mol.Biol., 342:31-41, 2004

PubMed Abstract: Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting. These studies require that naturally occurring enzymes be redesigned to recognize novel target sites. Here, we report studies in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains corresponding to contact points to distinct base-pairs in its target site. The resulting enzyme constructs drive specific elimination of selected DNA targets in vivo and display shifted specificities of DNA binding and cleavage in vitro. Crystal structures of two of these constructs demonstrate that substitution of individual side-chain/DNA contact patterns can occur with almost no structural deformation or rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and specificity.

PubMed: 15313605
DOI: 10.1016/j.jmb.2004.07.031

Experimental method

X-RAY DIFFRACTION (2.9 Å)