1TZL

Crystal Structure of Pyranose 2-Oxidase from the White-Rot Fungus Peniophora sp.

1TZL の概要

• エントリーDOI: 10.2210/pdb1tzl/pdb
• 分子名称: pyranose oxidase, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: gmc oxidoreductase, glucose-methanol-choline family, large inner cavity, oxidoreductase
• 由来する生物種: Peniophora sp. SG
• 細胞内の位置: Periplasm : Q8J136
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 566547.84

構造登録者

Bannwarth, M.,Bastian, S.,Heckmann-Pohl, D.,Giffhorn, F.,Schulz, G.E. (登録日: 2004-07-10, 公開日: 2004-10-19, 最終更新日: 2024-11-20)

主引用文献

Bannwarth, M.,Bastian, S.,Heckmann-Pohl, D.,Giffhorn, F.,Schulz, G.E.
Crystal structure of pyranose 2-oxidase from the white-rot fungus peniophora sp.
Biochemistry, 43:11683-11690, 2004

PubMed Abstract: Pyranose 2-oxidase catalyzes the oxidation of a number of carbohydrates using dioxygen. The enzyme forms a D(2) symmetric homotetramer and contains one covalently bound FAD per subunit. The structure of the enzyme from Peniophora sp. was determined by multiwavelength anomalous diffraction (MAD) based on 96 selenium sites per crystallographic asymmetric unit and subsequently refined to good-quality indices. According to its chain fold, the enzyme belongs to the large glutathione reductase family and, in a more narrow sense, to the glucose-methanol-choline oxidoreductase (GMC) family. The tetramer contains a spacious central cavity from which the substrate enters one of the four active centers by penetrating a mobile barrier. Since this cavity can only be accessed by glucose-sized molecules, the enzyme does not convert sugars that are part of a larger molecule. The geometry of the active center and a comparison with an inhibitor complex of the homologous enzyme cellobiose dehydrogenase allow the modeling of the reaction at a high confidence level.

PubMed: 15362852
DOI: 10.1021/bi048609q

実験手法

X-RAY DIFFRACTION (2.35 Å)