1TWC

RNA polymerase II complexed with GTP

1TWC の概要

• エントリーDOI: 10.2210/pdb1twc/pdb
• 関連するPDBエントリー: 1twa 1twf 1twg 1twh
• 分子名称: DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerases I, II, and III 7.7 kDa polypeptide, ZINC ION, ... (14 entities in total)
• 機能のキーワード: transcription, mrna, multiprotein complex, molecular machine, zinc motifs
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P08518 P16370 P20434 P20436 P27999 P38902; Nucleus, nucleolus: P40422 P22139; Cytoplasm: P20435
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 470782.31

構造登録者

Westover, K.D.,Bushnell, D.A.,Kornberg, R.D. (登録日: 2004-06-30, 公開日: 2004-11-16, 最終更新日: 2023-08-23)

主引用文献

Westover, K.D.,Bushnell, D.A.,Kornberg, R.D.
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center.
Cell(Cambridge,Mass.), 119:481-489, 2004

PubMed Abstract: Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.

PubMed: 15537538
DOI: 10.1016/j.cell.2004.10.016

実験手法

X-RAY DIFFRACTION (3 Å)